High-Resolution Melting for Accurate Assessment of DNA Methylation

High-resolution melting for accurate assessment of DNA methylation.

Methylation of cytosine in DNA is an epigenetic mark that is important for genome stability, transcriptional regulation of endogenous genes, and permanent silencing of transposable elements and viral sequences. Methylcytosine, sometimes referred to as the 5th base of DNA, constitutes 3%–6% of all cytosines in the human genome and occurs almost exclusively within the context of CpG dinucleotides...

Methylation-sensitive high resolution melting (MS-HRM): a new approach for sensitive and high-throughput assessment of methylation

In this article, we show that high resolution melting analysis (HRM) is a sensitive and specific method for the detection of methylation. Methylated DNA and unmethylated DNA acquire different sequences after bisulphite treatment resulting in PCR products with markedly different melting profiles. We used PCR to amplify both methylated and unmethylated sequences and assessed HRM for the determina...

High quality assessment of DNA methylation in archival tissues from colorectal cancer patients using quantitative high-resolution melting analysis.

High-resolution melting (HRM) analysis is a novel tool for analysis of promoter methylation. The aim of the present study was to establish and validate HRM analysis for detection of promoter methylation on archival formalin-fixed paraffin-embedded tissues from colorectal cancer patients. We first evaluated HRM assays for O(6)-methylguanine-DNA methyltransferase (MGMT) and adenomatous polyposis ...

rapid quantitative determination of gstp1 promoter methylation level using differential high resolution melting analysis (d-hrma)

quantitative evaluation of dnmt3b promoter methylation in breast cancer patients using differential high resolution melting analysis

dna methylation plays an important role in carcinogenesis through epigenetic silencing of tumor suppressor genes. aberrant methylation usually results from changes in the activity of dna methyltransferases (dnmts). some studies show that the overexpression of the dnmts may lead to aberrant methylation of tumor suppressor genes. also the overexpression of dnmts may be related to methylation stat...